ALSENSE – Detection of SARS-CoV-2
Real-Time ALSENSE-SARS-CoV-2-RT-qPCR PCR kit for the qualitative detection of SARS-CoV‑2 RNA by one-step real-time fluorescent reverse transcription PCR technology in a nucleic acid preparations isolated from clinical samples.
Product Details
The functional purpose of the Real-Time PCR kit ALSENSE-SARS-CoV-2-RT-qPCR: it is designed especially for clinical laboratory diagnostics and research and is used for the qualitative determination of 2019-nCoV coronavirus RNA in clinical samples.
The working principle is based on a one-step real-time fluorescent reverse transcription PCR technology.
The purpose of the Real-Time PCR kit ALSENSE-SARS-CoV-2-RTqPCR is to use it when 2019-nCoV coronavirus infection is suspected (if you have symptoms like fever, fatigue, loss of appetite, cough (dry), sore throat, headache, or shortness of breath).
Sensitivity. The sensitivity is 100 copies/ml and five genomic equivalents in reaction.
The ALSENSE kit detects the following genes:
- 3 loci of the 2019-nCoV coronavirus ORF1ab gene and 2019-nCoV coronavirus S, E, and N genes.
The kits can process either 48 or 96 clinical samples. The package of Real-Time PCR kit ALSENSE-SARSCoV-2-RT-qPCR consists of all necessary reagents for RT-PCR, including internal control, negative control, and positive control.
| Release form | Reagent | Volume | Amount |
| Release form 4, for 48 assays | ALSENSE reaction buffer | 0.75 mL | 1 tube |
| ALSENSE RT-polymerase | 0.06 mL | 1 tube | |
| PSC | 0.1 mL | 1 tube | |
| NSC | 0.5 mL | 1 tube |
| Release form 5, for 96 assays (auto) | ALSENSE reaction buffer | 1.5 mL | 1 tube |
| ALSENSE RT-polymerase | 0.12 mL | 1 tube | |
| PSC | 0.1 mL | 1 tube | |
| NSC | 0.5 mL | 1 tube |
The application takes place in clinical laboratory diagnostics and in research practice for the qualitative determination of viral RNA in clinical samples (the samples can be: nasal secret, nasopharyngeal swab, oropharyngeal swab, sputum, endotracheal aspirate, bronchoalveolar rinse, rectal smear, plasma, human blood serum). This process takes place in specialized medical services and healthcare institutions.
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