We are offering the Real Time RT-PCR Kit for diagnosis of SARS-Cov2 – the complete solution for your laboratory, no matter whether you choose a Manual or Automatic Extraction method.
RNA / DNA Extraction with AlgimedPREP – Magnetic Bead Extraction
Why magnetic bead extraction?
- High degree of RNA/DNA purification
- High RNA/DNA yield
- Stability of extraction
- Reduced time spent
- Automation capability
- Can be used with Rosche , Thermo, PerkinElmer and others
AlgimedPREP kit contents:
- Lysis buffer
- Wash buffer №1
- Wash buffer №2
- Elution buffer
Optional: plates, tips, sealing membrane
Kit contents additional components (depending on the kit):
- Tubes with internal control sample (ICS)
- Tips with negative control sample (NCS)
- Tubes 0,2 ml , 1,5 ml , 2,0 ml)
- Amplification plate
- Extraction plate including tips
- Magnetic rack
- Tubes with transport medium
- Sealing membrane
- Sterile swabs
| Manual Method | Semi-automatic Method | Automatic Method |
|---|---|---|
| Extraction time: 96 samples - 90-120 min | Extraction time: 96 samples - 60-70 min | Extraction time: 96 samples - 35 min |
| The probability of error (human factor) - extremely high | The probability of error (human factor) - average | The probability of error (human factor) - extremely low |
| Dependence on the supply of consumables (interruptions in supplies from local suppliers are possible) | Dependence on deep well plates supplies (interruptions in supplies from local suppliers are possible) | No dependence on the supply of consumables |
| High demand for application support | Average demand for application support | Low demand for application support |
Manual Method
Semi-automatic Method
Automatic Method
AMPLIFICATION with ALSENSE
ALSENSE kit includes:
- RT polymerase and reverse transcriptase
- Reaction buffer ALSENSE (includes internal control sample)
- NCS negative control sample
- PCS positive control sample
Sensitivity:
5 genomic equivalent per PCR reaction; from 100 copies RNA in the sample
Quality control through commercial controls:
- Bio-Rad controls
- Integrated DNA Technologies (IDT) controls
ALSENSE kits are adapted for the following devices:
Otherwise write to us!!!
CFX-96 (Bio Rad)
Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000, (Qiagen / Corbett)
DTlite, DTprime, DT-96, DT-48
